Current Issue : April-June Volume : 2020 Issue Number : 2 Articles : 9 Articles
Atazanavir sulfate act as a protease inhibitor for the treatment HIV infection. Drug is of crucial importance to treatment and related research all over the world. So it is great interest of study to overview the analytical profile of ATV alone and in combination. The significant work is already reported for estimation of ATV. The privilege applicability of ATV was found by HPLC method and subsequently UV method. Very less literature was reported by UPLC and HPTLC methods. The ATV found in combination with ritonavir and cobicistat which are mostly used. The present article attempt to review many existing methodologies and the instrumental conditions that were applied in the last decade to quantify ATV and its metabolites. The authors have made special efforts to gather and compile the brand names of ATV alone and in combination. Such knowledge will assist in the development of new delivery systems in pharmaceutical research. The investigation will be useful and beneficial pre-formulation research and further analytical study of ATV....
A novel, precise, sensitive and accurate UV spectrophotometric method has been developed for the estimation of azathioprine in bulk and tablet dosage form. UV 1800 double beam UV visible spectrophotometer with the pair of 10 mm path length matched Quartz cells were used for the study. The azathioprine shows the maximum absorbance at 280 nm in distilled water. Beerâ??s law was obeyed at the concentration range of 1-6 mcg/ml with the correlation coefficient of 0.9994. A recovery study was found to be 98.8-100.49. The %RSD values are less the 2 in accordance to the ICH guidelines. The LOD and LOQ were found to be 0.0974 and 0.2953 respectively. This method was validated in terms of accuracy, precision and ruggedness in accordance to the ICH guidelines. This method applied for the estimation of azathioprine tablets....
Simple, precise and accurate zero orders derivative spectroscopic method has been developed and validated for the estimation of Hibifolin. The drug shows maximum absorption at 664 nm in methanol solution and obeys Beerâ??s law in the concentration range of 50-250 microgram/ml. The linearity study carried and regression coefficient was found to be 0.9997 and it has showed good linearity, precision in this concentration range. The LOD and LOQ were found to be 2.6332 and 7.9014 microgram/ml. The % relative standard deviations were found less than 2. The method has been validated according to ICH guidelines for linearity, precision, ruggedness, LOD and LOQ. The developed and validated method can be successfully applied for reliable quantification of Hibifolin....
The present article describes about the development of zero order UV spectrophotometric method for the quantitative determination of valganciclovir hydrochloride in bulk and tablet dosage form. Validation study was performed to develop a simple, sensitive, rapid, accurate, and economical UV spectrophotometric method for the estimation of valganciclovir hydrochloride. UV 1800 double beam UV visible spectrophotometer with the pair of 10 mm path length matched Quartz cells were used for the study. Valganciclovir hydrochloride shows the maximum absorbance at 252 nm in distilled water. Beer’s law was obeyed at the concentration range of 1-6 µg/ml with the correlation coefficient of 0.9998. The regression equation was found to be Y=0.0379x-0.0039. The standard plot clearly shows a straight line passing through the origin. The percentage recoveries were found to be 99.99 to 100.72%. The percentage relative standard deviation was well within the range of <2%. The LOD and LOQ were found to be 0.1112 and 0.3372 µg/ml. The method was validated according to the ICH guidelines and it was applied successfully for the determination of valganciclovir hydrochloride tablets....
Two simple UV-spectrophotometric methods have been developed for estimation of trazodone HCl (TZH) in bulk and pharmaceutical formulation using zero order UV-spectrophotometry absorbance method (Method I) and zero order UV-spectrophotometry method using area under curve technique (Method II). Water was used as solvent for both methods. In water, trazodone hydrochloride showed λmax at 246.0 nm. AUC was integrated between two wavelengths 235.20 nm – 256.60 nm. In both methods trazodone showed linearity in the concentration range of 05-35 µg/ml (r2>0.99). Proposed methods were applied for oral dosage and amounts of TZH estimated by Method I were found to be 99%, by Method II were found to be 98.80% respectively. Both these methods were validated statistically and by recovery experiments....
Simple and rapid uv-spectrophotometric methods were investigated for pharmaceutical estimation of riociguat (RCG). RCG is a novel soluble guanylate cyclase (sGC) stimulator. We report here; zero order absorbance (Method I) and first order derivative UV-spectroscopy (Method II) for RCG. The analysis involved the use of methanol as solvent for determination of RCG using for both methods. The drug was found to be stable in aforementioned solvent for more than 72 hrs. Method I was executed by measuring absorbance at wavelength maximum of 321.45 nm while for Method II; post derivatization of zero order spectrum; the uniform area in between 329.00 - 346.60 nm was measured. The absorbance and the area were plotted against concentration respectively. The estimations obeyed the linearity range in between 5 - 25 microgram/ml. The mean correlation coefficient was found to be 0.999 after five replicate determinations of linearity of both developed methods. The non-interference of tablet excipients from the formulation (Rioteph) ensured the specificity for proposed methods. The accuracy of the methods was effectively reflected with a mean recovery of 99.70 (Method I) and 99.64 (Method II). The results for method precision were found to be within the specified limits; which is expressed as percentage RSD. The developed methods as based on measurement of both absorbance as well as the area after spectral derivatization found to be efficient. Both the developed methods were validated according to ICH guideline and proved statistically accurate, precise and sensitive; hence the studies can be extended effectively towards routine determination of RCG....
A novel, simple, precise and accurate area under curve spectroscopic method was developed and validated for the estimation of irbesartan in bulk and pharmaceutical dosage forms and AUC was measured at 202-212 nm in methanol and acetonitrile in ratio of 70:30. The linearity was found to be in the concentration range of 1-5 microgram/ml and the correlation coefficient was found to be 0.9991 and it has showed good linearity, reproducibility, precision in this concentration range. The regression equation was found to be Y=0.1163Ã?-0.007. The % recovery values were found to be within 98-100% showed that method was accurate. The LOD and LOQ were found to be 0.0273 and 0.08198 microgram/ml, respectively. The % RSD values were found less than 2. The developed method is accomplishing the validation parameters according to ICH guidelines. The developed method was successfully applied for the quantitative estimation of irbesartan in bulk and pharmaceutical dosage forms....
Zero order derivative spectroscopic method was developed and validated for the estimation of dexamethasone in bulk and pharmaceutical dosage forms. The absorption maximum was at 241 nm in 1N hydrochloric acid. The linearity was found to be in the concentration range 10-50 µg/ml and the correlation coefficient was found to be 0.999 and it has showed good linearity, reproducibility, precision in this concentration range. The regression equation was found to be Y=0.008 X + 0.004. The % recovery values were found to be within 99.65-100.46% showed that the method was accurate. The LOD and LOQ were found to be 0.5544 and 1.6632 mcg/ml, respectively. The % RSD values were less than 2. The present method was accomplish the validation parameters according to ICH guidelines like accuracy, precision, linearity, range, ruggedness, limit of detection and limit of quantization. The developed method was successfully applied for the quantitative estimation of dexamethasone in bulk and pharmaceutical dosage forms....
A simple, accurate and precise area under curve spectroscopic method was developed and validated for the estimation of dexamethasone in bulk and pharmaceutical dosage forms and AUC was measured at 236-246 nm in 0.1N HCl (hydrochloric acid). The linearity was found to be in the concentration range of 10-50 microgram/ml and the correlation coefficient was found to be 0.9992 and it has showed good linearity, reproducibility, precision in this concentration range. The regression equation was found to be Y =0.009 X + 0.007. The % recovery values were found to be within 98.74-100.8% showed that the method was accurate. The LOD and LOQ were found to be 0.101 and 0.304 respectively. The % RSD values were less than 2. The present methods were accomplishing the validation parameters according to ICH guidelines. The developed method was successfully applied for the quantitative estimation of dexamethasone in bulk and pharmaceutical dosage forms....
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